Mini Prep – Bacterial Cultures from 20120409 & 20120410

Isolated plasmid DNA from cultures inoculated on 20120409 (p16RK3-C) & 20120410 (p16RK3-A). These cultures finally produced bacteria. Slow growth was likely due to anaerobic conditions (sealed 15mL conical tubes) and slow agitation (on a rocker instead of a shaker). Isolated plasmid DNA using Qiagen Mini Prep Spin Kit according to manufacturer’s protocol. Eluted plasmid DNA with 30uL of Buffer EB and quantified on Roberts Lab NanoDrop1000.

Results:

p16RK3-A = 134.2ng/uL

p16RK3-C = 36.4ng/uL

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