Plasmid Isolation – Withering Syndrome Cultures from Yesterday

Isolated plasmid DNA from 3mL of each culture inoculated yesterday using QIAprep Spin Miniprep Kit (Qiagen) according to the manufacturer’s protocol. Eluted the plasmid DNA with 50uL of Buffer EB and spec’d on Roberts Lab NanoDrop1000.

Results:

Samples look fine; good yields, good OD260/280 ratios. Will perform NcoI restriction digests on samples to linearize them in preparation for use in a new standard curve.

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