Plasmid Quantification – NcoI Linearized Withering Syndrome Clones from 20120726

Performed sample quantification on the NcoI linearized clones from 20120726. Used 1uL of sample in 100uL of a 1:200 pico green dilution and quantified using the Teacan plate reader.

Results:

Concentrations (ng/uL):

pWC8 – 12.616

p16RK3 – 12.909

p16RK7 – 2.6673

p18RK7 – 26.091

The standard curve is below. Raw fluorescence data, sample concentrations and plate layout is here.

y = 664.74x + 215.97

d = 212.4

r = 0.99974

2 thoughts on “Plasmid Quantification – NcoI Linearized Withering Syndrome Clones from 20120726

  1. Pingback: qPCR – Promega 2x GoTaq Probe Master Mix Withering Syndrome Assay Limit of Detection | Sam's Notebook

  2. Pingback: qPCR – p18RK7 Curve IDT Primer Test | Sam's Notebook

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