qPCR – Withering Syndrome qPCR Assay Validation: Analytical Sensitivity Plate #2 of 3

Performed qPCR exactly as on 20120731; master mix calcs, plate layout, etc.

Immomix Lot#IMX-111A

Results:

qPCR Data File (CFX96)

qPCR Report (PDF)

Well, the results are not good; they’re poor, even. There’s a noticeable shift in sensitivity. All of the samples came up at nearly 1.5 – 2 Cqs later than they did on 20120731; including the positive control. I will repeat the run, so see if we see this again. The only change that has been made to this is that I had to open a new tube of Immomix than what I used previously and this new tube was from a different lot. Is there really a chance that we’re seeing lot-to-lot variation here???

I’ve added the Lot# used on 20120731 to that entry.

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