qPCR – Withering Syndrome qPCR Assay Sample Checks

Ran qPCR on water filter samples isolated on 20120127 in an attempt to find water samples that have a higher copy number than Nate’s existing samples for use in the withering syndrome qPCR assay validation.

Plate layout, cycling params, etc can be found in the qPCR Report (see Results).

Standard curve was the p16RK7 NcoI-linearized curve made on 20120730.

Baseline threshold was set to 400 and cycles to analyze was set to 41.

Results:

qPCR Data File (CFX96):Sam_2012-10-17 15-49-02_CC009827.pcrd

qPCR Report (PDF):Sam_2012-10-17 15-49-02_CC009827.pdf

Everything looked good. Will use sample TAF SD A2 as the high copy number water sample for the withering syndrome qPCR assay.

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