PCR – Withering Syndrome cPCR Assay Limit of Detection Test

Ran cPCR, but used Promega GoTaq Flexi instead of the 2x Immomix Taq (Bioline). Master mix calcs are here. cPCR recipe was provided by Lisa.

All samples run in duplicate.

Results:

Great, no amplification of any sort. More troubleshooting??!! Will begin troubleshooting tomorrow.

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