Repeated yesterday’s PCR, but utilized the p16RK7 plasmid curve (from 20120731) from 3e7 to 3e1. All samples run in duplicate.
Ladder is Hyperladder V (Bioline)
Only detectable amplification is in the highest concentration, 3e7 copies! On the positive side, at least the bands are neat and clean with no smearing. Holy crap, this