PCR – Withering Syndrome cPCR LoD Tests

Ran PCR to determine limit of detection using dilutions starting at 300 copies (the lowest detectable copy number shown on Nate’s poster).

Master mix calcs are here.

Results:

Ladder (Hyperladder V; Bioline) is loaded in furthest right lane. It is smeared due to a loading error by Sonja who loaded her sample in the same well as the ladder.

No amplification. Will repeat with higher copy number plasmid templates.

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