PCR – Withering Syndrome cPCR LoD Tests

Ran PCR starting with highest copy number of WS p16RK7 plasmid curve (from 20120731) due to lack of amplification of the 300 copy plasmid seen on 20130423.

Master mix calcs are here.

All samples were run in duplicate.

Results:

Ladder is Hyperladder V (Bioline).

Lowest copy number visible is 3e4 copies (30,000 copies). Will run subsequent PCRs with copy numbers between 30,000 and 3,000 to identify lower limit of detection for this withering syndrome cPCR assay.

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