Restriction Digest – Withering Syndrome Clone Plasmid (p16RK7) from 20131106

Performed restriction digest using NcoI. Reactions were run for 1hr @ 37C and then heat inactivated @ 65C for 20mins.

Each reaction contained:

  • Plasmid – 5uL
  • 10x NEB Buffer 3 – 5uL
  • NcoI – 1uL
  • H2O – 39uL

After inactivation, 5uL from each reaction were run on a 1% TBE gel to confirm digestion. 1uL of undigested plasmid was run along side the digest.

Results:

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