qPCR – Promega 2x GoTaq Probe Master Mix Withering Syndrome Assay Limit of Detection

Ran limit of detection qPCR for the Promega 2x GoTaq Probe Master Mix using p18RK7 (from 20120731) primary curve and p18RK7 low curve (from 20140507).

Master mix calcs are here: 20140506 – qPCR WSN1 Promega LoD-1

Cycling params, plate layout, etc. can be found in the qPCR Report in the Results below.

Results:

qPCR Report (PDF): Sam_2014-05-21 10-07-34_CC009827.pdf
qPCR Data File (CFX96): Sam_2014-05-21 10-07-34_CC009827.pcrd

Data can be found here: Promega Master Mix Withering Syndrome Limit of Detection

NOTE: Well B6 is an outlier. Without that sample, the R^2 changes from 0.977 to 0.990. The Cq value for this sample has been highlighted in its entry on the Promega Master Mix Withering Syndrome Limit of Detection spreadsheet.

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