Ran limit of detection qPCR for the Promega 2x GoTaq Probe Master Mix using p18RK7 (from 20120731) primary curve and p18RK7 low curve (from 20140507).
Master mix calcs are here: 20140506 – qPCR WSN1 Promega LoD-1
Cycling params, plate layout, etc. can be found in the qPCR Report in the Results below.
Data can be found here: Promega Master Mix Withering Syndrome Limit of Detection
NOTE: Well B6 is an outlier. Without that sample, the R^2 changes from 0.977 to 0.990. The Cq value for this sample has been highlighted in its entry on the Promega Master Mix Withering Syndrome Limit of Detection spreadsheet.