Ran qPCR on subset of Ab Endo 2011 water filters extracted on 20140822 using the withering syndrome qPCR assay. See Ab Endo Samples spreadsheet and/or the qPCR Report (see Results below) for which samples were qPCR’d.
Master mix calcs are here: 20140826 – qPCR 2011 Ab Endo Water Filters_01
All samples were run in duplicate.
Standard curve was p18RK7 from 20120731.
Used IDT probe.
Plate layout, cycling params, etc can be seen in the qPCR Report (see Results below).
qPCR Report (PDF): Sam_2014-08-26 16-31-58_CC009827.pdf
qPCR Data File (CFX96): Sam_2014-08-26 16-31-58_CC009827.pcrd
Threshold was set at 580 RFUs, based on an average of runs using Promega 2x Probe Master Mix by Lisa.
Well, this is not good. Curve looks bad. Only difference is using the IDT probe. But, there’s not sufficient ABI probe to handle the remainder of the samples. Will have to test out some things with the IDT probe…