The Tecan plate reader is behaving strangely. I tried to quantify plasmid DNA samples isolated on Friday, but this is how the plate turned out:
The areas NOT demarcated by the black lines should all appear as dark blue (i.e. no fluorescence detected). I’ve never seen a plate look like this before.
To be sure that the issue wasn’t specific to just the plate I was using, I ran an empty plate through the same Tecan method.
Here’s the pic of an entirely empty/blank plate (i.e. no samples loaded in any wells; just an empty, dry plate):
Ideally, these should all be the same exact color (dark blue), since there should be no fluorescence in any wells.
I probably need to contact Tecan regarding this issue…