In-situ Hybridization (ISH) – Abalone Withering Syndrome and Phage ORF25: Day 3

Slides were briefly rinsed in dH2O three times.

Slides were counter stained with 0.05% aqueous Bismark Brown Y for 3mins @ RT.

Slides were briefly rinsed in dH2O, then 70% EtOH, then 100% EtOH.

Slides were air-dried in the fume hood.

Coverslips were added to each slide with three drops of Permount.

Permount was allowed to dry O/N at RT.

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