Performed reverse transcription with 307ng of each DNased withering syndrome RNA from earlier today using Promega reagents (M-MLV, random primers) according to the manufacturer’s recommendations in the M-MLV product insert.
Reverse transcription calcs are here: 20150318 – WS Viability cDNA Calcs
Incubated DNased RNA/primer mixture @ 70C for 5mins. Placed immediately on ice. Added M-MLV master mix to each sample, incubated @ 37C 1hr and held at 4C in thermalcycler.