Reverse Transcription – Withering Syndrome DNased RNA

Performed reverse transcription with 307ng of each DNased withering syndrome RNA from earlier today using Promega reagents (M-MLV, random primers) according to the manufacturer’s recommendations in the M-MLV product insert.

Reverse transcription calcs are here: 20150318 – WS Viability cDNA Calcs

Incubated DNased RNA/primer mixture @ 70C for 5mins. Placed immediately on ice. Added M-MLV master mix to each sample, incubated @ 37C 1hr and held at 4C in thermalcycler.

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  1. Pingback: qPCR – Withering Syndrome Water Filter cDNA | Sam's Notebook

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