qPCR – Withering Syndrome Water Filter cDNA

Ran qPCR on withering syndrome water filter cDNA from yesterday.

Master mix calcs are here: 20150319 – qPCR WSN cDNA

Ran p18RK7 standard curve from 20120731.

All samples were run in duplicate.

Plate layout, cycling params, etc. can be found in the qPCR Report (see Results).

qPCR Report (PDF): Sam_2015-03-19 09-47-31_CC009827.pdf
qPCR Data File (CFX96): Sam_2015-03-19 09-47-31_CC009827.pcrd

Standard curve amplified properly and looks perfect.

No amplification in any other samples.

Will plan on verifying cDNA is actually good by (which I should have thought to include on this run) qPCRing with some universal bacterial primers.


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  1. Pingback: qPCR – Withering Syndrome cDNA Tests | Sam's Notebook

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