qPCR – Withering Syndrome Water Filter cDNA

Ran qPCR on withering syndrome water filter cDNA from yesterday.

Master mix calcs are here: 20150319 – qPCR WSN cDNA

Ran p18RK7 standard curve from 20120731.

All samples were run in duplicate.

Plate layout, cycling params, etc. can be found in the qPCR Report (see Results).

Results:
qPCR Report (PDF): Sam_2015-03-19 09-47-31_CC009827.pdf
qPCR Data File (CFX96): Sam_2015-03-19 09-47-31_CC009827.pcrd

Standard curve amplified properly and looks perfect.

No amplification in any other samples.

Will plan on verifying cDNA is actually good by (which I should have thought to include on this run) qPCRing with some universal bacterial primers.

 

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  1. Pingback: qPCR – Withering Syndrome cDNA Tests | Sam's Notebook

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