Ran a PCR using the new ISH primers that I previously designed:
Template DNA was black abalone DNA (from digestive gland [Dg]): 06:6-54 (from 4/9/2008)
Negative control DNA: UW08:22-11A (from 3/5/2007)
No template controls (NTCs) were also run.
All samples were run in duplicate, in 0.5mL PCR tubes.
Master mix calcs
|REAGENT||SINGLE REACTION (μL)||x6.6 (μL)|
|2x Apex Red Master Mix||12.5||82.5|
|TOTAL||25||Add 24μL to each tube|
Cycling Params (PTC-200; MJ Research)
|STEP||TEMP (C)||TIME (s)|
Samples were held O/N at 4C. Will run on gel tomorrow.