Standard Curve Dilutions – pCR2.1/RLOv Plasmids

Set up standard curve dilutions to use for qPCRs with the following two linearized plasmids:

  • pCR2.1/RLOv_DNA_helicase
  • pCR2.1/RLOv_head_to_tail

Used a spreadsheet developed by Nate many moons ago to determine necessary volumes based on plasmid size to obtain desired copy numbers.

Dilutions were made in TE Buffer.

DNA helicase dilutions (Google Sheet): 20151106 – Dilution table RLOv_DNA_helicase_qPCR_Standard_Curve

Head-to-tail dilutions (Google Sheet): 20151106 – Dilution table RLOv_head_to_tail_qPCR_Standard_Curve

2 thoughts on “Standard Curve Dilutions – pCR2.1/RLOv Plasmids

  1. Pingback: qPCR – RLOv DNA Helicase on Black Abalone | Sam's Notebook

  2. Pingback: qPCR – pCR2.1/RLOv Standard Curves Testing | Sam's Notebook

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