Yesterday’s check of the RLOv DNA helicase standard curve using a freshly made 3 copy sample didn’t look perfect, so I’m re-running to see if things will tighten up a bit.
Repeated yesterday’s run with no changes.
Ran qPCR using the RLOv DNA helicase standard curve from 20151106.
All samples were run in duplicate.
Master mix calcs are here (Google Sheet): 20151223 – qPCR RLOv DNA Helicase Curve Check
Plate layout, cycling params, etc. can be found in the qPCR Report (see Results below).
Overall, things are looking better than yesterday’s run: better reps, better efficiency and better R^2. Will move forward with beginning to validate this qPCR assay, as well as use for some other sample analysis that Carolyn has in mind (comparing RLOv vs. WS levels in abalone collected from wild sites in California).