This is a qPCR to test the fresh p18RK7 dilution I made earlier today, and verify it works well (i.e. linear spread, good R^2 value, same Cqs as previous curve dilution, etc.).
Master mix calcs (Google Sheet): 20150316 – qPCR WS p18RK7 Curve Check
I made three separate master mixes to check the curve three times.
All samples were run in duplicate. Plate layout, cycling params, etc. are in the qPCR Report (see Results below).
Baseline threshold was manually set to 580, as previously determined by Lisa for this assay.
Overall, the curve looks good and has very comparable Cq values at each dilution of the curve. Will use this for future withering syndrome qPCR assays and will send an aliquote of each dilution to Colleen Burge.
MASTER MIX #1
MASTER MIX #2
MASTER MIX #3