Isolated DNA from EtOH-preserved black abalone digestive gland tissue from the 2nd black abalone experiment.
There’s some odd background in regards to these samples which I previously described here that might be worth reviewing.
DNA was isolated using the QIAamp Fast DNA Stool Kit (Qiagen). Tissues were weighed and briefly homogenized with a disposable pestle in InhibitEX Buffer. Manufacturer’s protocol was followed. DNA was eluted in 100μL of Buffer ATE and quantified on the Roberts Lab Qubit3.0 (ThermoFisher) using 1μL with the Qubit dsDNA Broad Range assay.
Google Sheet: 20160421_DNA_isolation_08:13_subset