Checking DNA isolated earlier today from the 2nd black abalone experiment to see if withering syndrome (RLO) and/or the withering syndrome phage (RLOv) is detectable in these samples.

Master mix calcs

• WSN1: 20150421 – qPCR Black Ab 08:13 WSN1 Check
• RLOv DNA helicase: 20160421 – qPCR Black Abs RLOv DNA Helicase

Standard curves

• RLOv DNA helicase standard curve from 20151224.
• WSN p18RK7 standard curve from 20160316.

All samples were run in duplicate.

Plate layout, cycling params, etc. can be seen in the qPCR Report (see Results below).

Baseline thresholds were set to the following values for each assay (RLOv threshold determined by me on 20160128; WSN1 threshold determined by Lisa):

RLOv DNA helicase: 580.5

WSN1: 580

Results:

qPCR Report – RLOv DNA helicase (PDF): Sam_2016-04-21 12-39-33_CC009827_RLOv_DNA_helicase.pdf
qPCR Report – WSN1 (PDF): Sam_2016-04-21 12-39-33_CC009827_WSN.pdf
qPCR Data File (CFX): Sam_2016-04-21 12-39-33_CC009827.pcrd

RLOv DNA helicase does not amplify in any samples.

WSN1 amplifies in all samples.

All samples are RLO+/RLOv-, as seen in the previous set of 08:13 samples that I qPCR’d.

RLOv DNA Helicase Standard Curve

RLOv DNA Helicase Amplification (Green = Std Cuve, Blue = Samples)

WSN1 Standard Curve

WSN1 Amplification (Blue = Standard Curve, Magenta= Samples)