qPCR – Black Abalone with XC Prophage Portal Primers

I accidentally skipped two samples from the 2nd black abalone experiment sample set that I qPCR’d last week, so I’m qPCRing them today.

Master mix calcs (Google Sheet): 20160425 – qPCR Black Abs XenoCal phage portal

All samples were run in duplicate.

Plate layout, cycling params, etc. can be seen in the qPCR Report (see Results below).

Results:
qPCR Report (PDF): Sam_2016-04-25 12-55-40_CC009827.pdf
qPCR Data File (CFX96): Sam_2016-04-25 12-55-40_CC009827.pcrd

Have amplification in both samples.

I will add this to a “master” spreadsheet that I’ve made containing qPCR data from three genes on ~20 samples from both the 1st and 2nd black abalone experiments.

 

 

 

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