I accidentally skipped two samples from the 2nd black abalone experiment sample set that I qPCR’d last week, so I’m qPCRing them today.
Master mix calcs (Google Sheet): 20160425 – qPCR Black Abs XenoCal phage portal
All samples were run in duplicate.
Plate layout, cycling params, etc. can be seen in the qPCR Report (see Results below).
Have amplification in both samples.
I will add this to a “master” spreadsheet that I’ve made containing qPCR data from three genes on ~20 samples from both the 1st and 2nd black abalone experiments.