Continued preparation of this RNA to assess withering syndrome viability in the water column. I treated the RNA I isolated on 20161130 using the Turbo DNA-free (Ambion) DNase kit, according to their protocol.

Added the following to each sample:

• 2.5μL 10x buffer
• 1.5μL H2O
• 1μL DNase

Incubated @ 37C for 1hr.

Added 0.1 volumes (2.5μL) of DNase Inactivation reagent and incubated at RT for 2mins (with mixing). Pelleted inactivation reagent: 10,000g, 2mins, RT. Transferred supe to new tube.

Samples were labelled as “DNased RNA”, their existing sample name (see below), and stored @ -80C.

Sample names:

• T0A
• T0B
• T1A
• T1B
• T1A C
• T1B C
• T3A
• T3B
• T7A
• T7B
• T7A C
• T7B C