Continued preparation of this RNA to assess withering syndrome viability in the water column. I treated the RNA I isolated on 20161130 using the Turbo DNA-free (Ambion) DNase kit, according to their protocol.
Added the following to each sample:
- 2.5μL 10x buffer
- 1.5μL H2O
- 1μL DNase
Incubated @ 37C for 1hr.
Added 0.1 volumes (2.5μL) of DNase Inactivation reagent and incubated at RT for 2mins (with mixing). Pelleted inactivation reagent: 10,000g, 2mins, RT. Transferred supe to new tube.
Samples were labelled as “DNased RNA”, their existing sample name (see below), and stored @ -80C.
- T1A C
- T1B C
- T7A C
- T7B C