Prior to creating cDNA, need to verify that the DNased RNA from earlier today doesn’t contain any detectable RLO DNA.
Master mix calcs (Google Sheet): 20161128 – qPCR Water Filter DNased RNA
All samples were run in duplicate. Plate layout, cycling params, etc. are in the qPCR Report (see Results below).
Standard curve was the p18RK7 curve made on 20161128.
Baseline threshold was manually set to 580, as previously determined by Lisa for this assay.
Standard curve looks good.
No samples amplified. This suggests that there is no detectable DNA in any of the DNased RNA samples. Will proceed with making cDNA.