The RNA I isolated earlier today was subjected to DNase treatment using the Turbo DNA-free Kit (Invitrogen), following the manufacturer’s standard protocol.
After DNase inactivation treatment, the RNA was transferred (recovered ~19uL from each samples) to a clear, low-profile PCR plate.
The plate layout is here (Google Sheet): 20170309_RLO_viability_DNased_RNA_plate_layout
The samples will be subjected to qPCR to assess the presence/absence of residual gDNA. The plate of DNased RNA was stored @ -80C in the original box that the water filters were stored in.
An overview of the experiment and the various treatments are viewable in the “Viability Trial 2″ tab of Lisa’s spreadsheet (Google Sheet): RLO Viability & ID50