Water filters stored at -80C in ~1mL of RNAzol RT were provided by Lisa. This is part of an experiment (and Capstone project) to assess RLO viability outside of the host.
The samples were thawed and briefly homogenized (as best I could) with a disposable plastic pestle. The samples were then processed according to the manufacturer’s protocol for total RNA isolation. Samples were resuspended in 25μL of nuclease-free water (Promega).
The experimental samples and the various treatments are viewable in the “Viability Trial 3″ tab of Lisa’s spreadsheet (Google Sheet): RLO Viability & ID50