Ran qPCRs on a set of black abalone digestive gland DNA (sample list provided by Carolyn):
07:12-01 (Black Ab exp 1)
07:12-02 (Black Ab exp 1)
08:13-05 (Black Ab exp 2)
08:13-18 (Black Ab exp 2)
08:13-24 (Black Ab exp 2)
08:13-25 (Black Ab exp 2)
UW06:06-50 (Black Ab exp 1)
UW06:06-52 (Black Ab exp 1)
The two samples with a strikethrough did not have any DNA left in the tubes and were not run.
All samples were run in duplicate.
Standard curve was from 20161106.
Cycling params, plate layout, etc can be seen in the qPCR Report (see Results).
Baseline was set 580.5 as previously determined.
qPCR Report (PDF): Sam_2017-04-13%2016-20-54_CC009827_RLOv_helicase.pdf
qPCR Data File (CFX): Sam_2017-04-13%2016-20-54_CC009827_RLOv_helicase.pcrd
Standard curve looked good, although efficiency is pushing it on the high end.
The following samples did <em>not</em> amplify:
- All 08 samples.
The remaining samples all came up positive, with the 06 set being extremely hot (came up around cycle 13).
Will convey to Carolyn and Stan.