DNA Extraction – Ava Withering Syndrome Transmission Study Tissues

Isolated DNA from five tissue samples.

Tissue was weighed and then DNA extracted.

DNA was extracted using the QIAmp Fast DNA Stool Mini Kit (Qiagen) following the manufacturer’s protocol with the following options:

  • Samples were briefly homogenized (due to their stiffness resulting from ethanol fixation) in the InhibitEX Buffer using disposable plastic pestles.
  • Homogenized tissue was incubated at 95C to maximize cell lysis
  • Followed “human DNA analysis” protocol for remainder of protocol (to maximize sample recovery)
  • Eluted DNA with 100μL Buffer ATE

Samples were stored at 4C in FSH240 in racks for qPCR and quantification later today.

Sample information is in this spreadsheet (Google Sheet): 20170502_Ava_Ab_List

2 thoughts on “DNA Extraction – Ava Withering Syndrome Transmission Study Tissues

  1. Pingback: qPCR – Ava’s RLO Transmission Samples | Sam's Notebook

  2. Pingback: DNA Quantification – Ava’s RLO Transmission DNA | Sam's Notebook

Leave a Reply

Your email address will not be published. Required fields are marked *

e.g. 0000-0002-7299-680X

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>