Isolated DNA from 19 red abalone digestive gland tissue samples.
Tissue was weighed and then DNA extracted.
DNA was extracted using the QIAmp Fast DNA Stool Mini Kit (Qiagen) following the manufacturer’s protocol with the following options:
- Samples were briefly homogenized (due to their stiffness resulting from ethanol fixation) in the InhibitEX Buffer using disposable plastic pestles.
- Homogenized tissue was incubated at 95C to maximize cell lysis
- Followed “human DNA analysis” protocol for remainder of protocol (to maximize sample recovery)
- Eluted DNA with 100μL Buffer ATE
Samples were stored at 4C in FSH240 in racks for qPCR later today.
Sample information is in this spreadsheet (Google Sheet): Ava WS Transmission DNA Extractions