The withering syndrome standard curve on my last two qPCRs has been wonky, so I’m making a new curve.
Used the 3e7 dilution of p18RK7 from 20161128 as the “stock” to make serial, 1:10 dilutions (final volume of 1000uL in each tube).
I prepared the dilution series using IDTE (pH=8.0; IDT) Buffer. The original dilution worksheet below was set up by Nate Wight many, many, moons ago.
Dilution calcs (Google Sheet): 20160316 – WS p18RK7 plasmid dilution table
Will test out the new curve dilution shortly.