Sample Prep – Withering Syndrome p18RK7 Plasmid Standard Curve Dilution

The withering syndrome standard curve on my last two qPCRs has been wonky, so I’m making a new curve.

Used the 3e7 dilution of p18RK7 from 20161128 as the “stock” to make serial, 1:10 dilutions (final volume of 1000uL in each tube).

I prepared the dilution series using IDTE (pH=8.0; IDT) Buffer. The original dilution worksheet below was set up by Nate Wight many, many, moons ago.

Dilution calcs (Google Sheet): 20160316 – WS p18RK7 plasmid dilution table

Will test out the new curve dilution shortly.

2 thoughts on “Sample Prep – Withering Syndrome p18RK7 Plasmid Standard Curve Dilution

  1. Pingback: Sample Prep – Withering Syndrome p18RK7 Plasmid Standard Curve Dilution | Sam's Notebook

  2. Pingback: qPCR – Check New Withering Syndrome p18RK7 Plasmid Standard Curve Dilution | Sam's Notebook

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