Sample Prep – Withering Syndrome p18RK7 Plasmid Standard Curve Dilution

The last dilution I made was jacked up, so I made a new one today.

Used the 3e7 dilution of p18RK7 from 20161128 as the “stock” to make serial, 1:10 dilutions (final volume of 1000uL in each tube).

I prepared the dilution series using lab-made Low TE Buffer (pH=8.0). The original dilution worksheet below was set up by Nate Wight many, many, moons ago.

Dilution calcs (Google Sheet): 20160316 – WS p18RK7 plasmid dilution table

Will test out the new curve dilution shortly.

One thought on “Sample Prep – Withering Syndrome p18RK7 Plasmid Standard Curve Dilution

  1. Pingback: qPCR – Check New Withering Syndrome p18RK7 Plasmid Standard Curve Dilution | Sam's Notebook

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