DNA Isolation – Ava Withering Syndrome Transmission Study Tissues

Isolated DNA from 144 red abalone digestive gland tissue samples.

Tissue was weighed, minced with a razor blade, and transferred to 2mL snap cap tube containing 1mL of InhibtEX Buffer.

DNA was extracted using the QIAmp Fast DNA Stool Mini Kit (Qiagen) following the manufacturer’s protocol with the following options:

Minced tissue was incubated at 70C O/N
Followed “human DNA analysis” protocol (to maximize sample recovery)
Eluted DNA with 100μL Buffer ATE
Sample information is in this spreadsheet (Google Sheet): ava_abalone_master_extraction_list

One thought on “DNA Isolation – Ava Withering Syndrome Transmission Study Tissues

  1. Pardon us, Sam,

    This is our final test of new code which sends you an email per each new comment on one of your notebooks. This will not affect your moderation settings; e.g., a user such as yourself or Steven will have comments posted automatically and you will receive an email accordingly. That is, for this code to run more liberally, modify discussion settings to publish comments more liberally.

    This comment is on Sam’s Notebook – Friedman Lab and we will run the test on them both.

Leave a Reply

Your email address will not be published. Required fields are marked *

e.g. 0000-0002-7299-680X

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>