Isolated DNA from the following pinto abalone (Haliotis kamtschatkana) digestive gland tissues (stored in ethanol), collected by Sean Bennett as part of his Capstone project:
| Accession | Weight(mg) |
|---|---|
| 15:30-01 | 194 |
| 15:30-04 | 67 |
| 15:31-01 | 34 |
| 15:31-02 | 107 |
| 15:31-03 | 83 |
| 15:31-04 | 80 |
Tissue was weighed and then DNA extracted.
DNA was extracted using the QIAmp Fast DNA Stool Mini Kit (Qiagen) following the manufacturer’s protocol with the following options:
- Samples were briefly homogenized (due to their stiffness resulting from ethanol fixation) in the InhibitEX Buffer using disposable plastic pestles.
- Homogenized tissue was incubated at 95C to maximize cell lysis
- Followed “human DNA analysis” protocol for remainder of protocol (to maximize sample recovery)
- Eluted DNA with 100μL Buffer ATE
Used the Roberts Lab Qubit 3.0 and the Qubit hsDNA Kit (high sensitivity). Used 1uL of template for all samples.
Samples were stored at -20C in FSH240 in the “Pinto Transcriptome DNA” box.
Results:
All samples have DNA.
Concentrations (Google Sheet): 20171226_qubit_DNA_pinto_ab