Quick withering syndrome qPCR assay test using the ABI RLP_p probe and IDT primers. This is to see if we can still use IDT primers that have NOT been ABI HPLC purified. This would be a significant cost savings, as HPLC purified primers from ABI cost ~$60 each (“regular” IDT primers only cost ~$6 each). This should work.
Master mix calcs are here: 20140502 – qPCR p18RK7 Curve WSN1 IDT Primers
Used p18RK7 curve from 20120730 because we are running low on the p16RK7 curve (from the same date).
Plate layout, cycling params, etc can be found in the qPCR Report in the Results below.
Not surprising,the IDT primers work just fine. Will continue to use/order IDT primers for withering syndrome PCRs.