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University of Washington – Fishery Sciences – Friedman Lab

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Category Archives: Samples Submitted

Sanger Sequencing – pCR2.1/OsHV-1 ORF117

Posted on August 17, 2017 by kubu4
1
0000-0002-2747-368X

Submitted plasmid DNA isolated earlier today to Genewiz for Sanger sequencing using the M13F/R primers.

Sequenced each of the two plasmids three times with each forward and reverse primer. Here’s a copy of the sample submission form:

Posted in Samples Submitted | Tagged GENEWIZ, OsHV ORF117, OsHV-1, pCR2.1, plasmid, Sanger sequencing | 1 Reply

Sample Submission – Pinto Abalone Tissue for Histology Processing

Posted on July 21, 2017 by kubu4
Reply
0000-0002-2747-368X

Tracked down and submitted four cassettes containing pinto abalone digestive gland tissue that Sean Bennett prepared, as part of his Capstone project. A full list of samples from his project is here (Google Sheet): Pinto Transcriptome

Sent them to Diagnostic Pathology Medical Group for histology processing.

 

 

 

Posted in Samples Submitted | Tagged 15:30-1, 15:30-2, 15:30-3, 15:30-4, 15:30-5, 15:30-6, 15:31-1, 15:31-2, 15:31-3, 15:31-4, 15:31-5, Haliotis kamtschatkana, histology, pinto abalone, Sean Bennett | Leave a reply

Samples Submitted – Pinto Abalone RNA & Tissues to UC-Irvine

Posted on February 22, 2017 by kubu4
1
0000-0002-2747-368X

Shipped pinto abalone tissues and RNA to Alyssa Braciszewski at UC-Irvine. These were collected by Sean Bennett during his Capstone Project.

I prepped samples earlier today: http://onsnetwork.org/sjwfriedmanlab/2017/02/22/sample-prep-pinto-abalone-tissuerna-for-collabs-at-uc-irvine/

A full list of samples shipped is here (Google Sheet): 20170222_pinto_abalone_samples

Samples were shipped O/N on dry ice via FedEx: 778526365337

Posted in Samples Submitted | Tagged Haliotis kamtschatkana, pinto abalone | 1 Reply

MiSeq Sequencing Submission – Withering Syndrome Variants DNA

Posted on June 4, 2015 by kubu4
Reply
0000-0002-2747-368X

The following DNA samples were isolated from red abalone digestive gland by Lisa on 20150016 :

  • 14-5-2 (RLO Only)
  • 14-5-3 (Stippled)

Prior to submission, I quantified the DNA on the Roberts Lab NanoDrop1000.

Results:

I submitted 3μg of each sample to Stan Langevin at the University of Washington Department of Microbiology for sequencing on the Illumina MiSeq, and subsequent analysis.

Posted in Abalone Withering Syndrome Characterization, Samples Submitted | Tagged 14-5-2, 14-5-3, 14:5-2, 14:5-3, Dg, digestive gland, DNA, DNA quantification, Haliotis rufescens, MiSeq, NanoDrop1000, red abalone, RLO, Stan Langevin, stippled, withering syndrome, WS | Leave a reply

Sample Submission – Black Ab DNA for MiSeq by Stan Langevin

Posted on July 18, 2014 by kubu4
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0000-0002-2747-368X

Submitted 100ng of each of the following DNA samples that were quantified yesterday to Stan Langevin for sequencing on the Illumina MiSeq to attempt to sequence the RLO phage:

  • 06:5-28
  • 06:6-41
  • 06:6-44
  • 06:6-53
  • 06:6-54
  • 06:6-55
  • 06:6-66
Posted in Abalone Withering Syndrome Phage ID, Samples Received, Samples Submitted | Tagged 06:5-28, 06:6-41, 06:6-44, 06:6-53, 06:6-54, 06:6-55, 06:6-66, black abalone, Haliotis cracherodii, phage, withering syndrome, WS | Leave a reply

Projects

  • Ab Endo (30)
    • 2010 Samples (16)
    • 2011 Samples (16)
  • Abalone Withering Syndrome Characterization (13)
  • Abalone Withering Syndrome Phage ID (72)
    • In-situ Hybridization (23)
    • qPCR Standard Curve (15)
    • RLOv DNA Helicase qPCR Assay Validation (8)
  • Abalone Withering Syndrome qPCR Assay Validation (100)
    • Abalone Withering Syndrome qPCR Troubleshooting (70)
  • Ava's Abalone RLO Transmission Experiment (33)
  • Cg OA Selection (1)
  • Computer Servicing (8)
  • Computing (2)
  • Coral RLO (4)
  • Dan's Multi-Family Oyster Crosses Project (7)
  • Ireland Clam RLO ID (16)
  • Lab Maintenance (6)
  • Miscellaneous (127)
  • OA Lesson Plan (3)
  • Samples Received (14)
  • Samples Submitted (5)

Tags

abalone black abalone CFX96 Crassostrea gigas Dg digestive gland DNA DNA isolation DNA quantification DNased RNA DNeasy gel GoTaq qPCR Master Mix GoTaq qPCR Probe Master Mix Haliotis cracherodii Haliotis rufescens Hyperladder I Immomix in-situ hybridization NanoDrop1000 OsHV ORF117 p16RK3 p16RK7 p18RK7 Pacific oyster PCR pCR2.1 PCR validation phage pics QIAamp Fast DNA Stool Mini Kit qPCR qPCR standard curve Quant-iT BR Qubit 3.0 red abalone RLO RLOv RLOv_DNA_helicase SPUD troubleshooting water filters withering syndrome WS WSN1

Recent Posts

  • Equipment Maintenance – Alarm Battery Replacements
  • qPCR – Pinto Abalone DNA with WSN1 and RLOv DNA Helicase
  • Cloning – Purified OsHV-1 ORF117 PCRs
  • DNA Isolation & Quantification – Pinto Abalone
  • PCR – OsHV-1 ORF117 from Australian, California, & French Variants

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