De-paraffinization – Abalone PE from OTC15-250 for DNase Tests

Performed de-paraffinization according to the Friedman Lab protocol on 0.0185g of thinly sliced tissue from the tissue above in a single 5mL snap-cap tube. Briefly, tissue was treated as follows:

  • SafeClear II – 3mins x 3
  • 100% EtOH – 3mins x 2
  • 95% EtOH – 3mins x 2
  • 80% EtOH – 3mins x 2
  • 70% EtOH – 3mins x 2
  • 50% EtOH – 3mins x 2

Sample was left in 50% EtOH at RT until ready for further processing.