Samples Received – Green Abalone Slides

Received green abalone (Haliotis fulgens) slides from Fiorenza Micheli (Hopkins Marine Station, Stanford University). These are related to the tissue she sent to us on 20160519.

Unfortunately, the writing on the casettes that were submitted to DPMG came off during processing (see note on form below). As such, the facility was unable to identify which slides came from which histology blocks. Not sure what we’ll be able to do with these. The box of slides has been stored in FSH 236 with other slide boxes.

DNA Isolation – Stool DNA Extraction from Sea Lab Green Abalone

Isolated DNA using the Qiagen Stool Kit. Two samples were provided from Sea Lab:

  1. Dead, rotten green abalone

  2. Green abalone fecal sample in ~50mL sea water

According to the documentation provided by Sea Lab, the abalone died, was refrigerated (didn’t tell us how long), then frozen, then sent to us via overnight shipping on wet ice. Here’s a picture of the abalone (colors have not been altered!):

The only remaining identifiable morphology was the radula. Otherwise, the grey color and the mushy consistency persisted through the entire “animal”. As I was hoping to take a sample from the post-esophagus (for eventual withering syndrome detection) and there were no discernable morphologies, I opted to take 200uL of the black liquid surrounding the abalone from the bag it came in.

For the fecal sample, the feces were pelleted (2500g, 20mins, 4C), seawater discarded and pellet weighed (72.5mg).

Both samples were isolated using Qiagen’s Stool Kit according to protocol.

UPDATE 20140814

Sean Bennett ran a qPCR on both samples, each in duplicate. They are on his plate labeled as “AB” and “Feces”. They are located in wells A3, A4 and B3, B4, respectively. Both samples came up positive, with the abalone tissue sample coming up at ~34 Cqs and the fecal sample coming up at ~26 Cqs. The standard curve on his plate didn’t work well, so a quantity can’t be accurately determined from this run.