Performed reverse transcription using 5ug of total DNased RNA from yesterday following Promega’s MMLV RT and Oligo dT primers according to Promega’s protocol.
Performed reverse transcription of 5 black abalone Dg DNased RNA (from 20090421) according to Promega’s recommendations using M-MLV reverse transcriptase. Calculations are here.
DNased RNA Samples Used
In order to have sufficient cDNA to use for primer optimization, the 07:12-17 and the 08:3-6 cDNA samples were pooled.
Samples were stored @ -20C in Sam’s cDNA Box #2 (Roberts Lab).