Tag Archives: Olympia oyster

Morphometrics – Olympia Oyster OA Larvae Completed

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Finished measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software). That’s 2,620 larvae measured!

NOTE: Robyn previously measured 33 tubes worth of larvae, but I’m not certain where that data was saved.

All measurements by me can be found here (Google Sheet): FHL_Oly_larvae_measurements

Measured 30 larvae from each tube of the following samples:

  Box Label Color Tube Label Notes
70 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 U FB HL.L-1
71 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 V FB HL.L.2
72 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 W FB HH.L.1
73 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 X FB HH.L.2
74 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 Y SS LLH3 Many transluscent shells
75 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 Z SS LL.H.4 Many transluscent shells
76 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 AA SS LHH3 Mostly transluscent shells
77 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 BB SS LHH4
78 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 CC SS LL.L.3
79 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 DD SS LL.L.4
80 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 EE SS LH.L.3
81 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 FF SS LH.L.4
82 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LLL-1 4.3.E
83 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LLL2 4.3.F
84 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LHL-1 4.3.G
85 FHL Oly OA x DO Box 2 etOH Friedman Pink 4.4.15 FB release Low CO2 103A
86 FHL Oly OA x DO Box 2 etOH Friedman White SS Low CO2 4.4.15 release 103A All shells partially transluscent.
87 FHL Oly OA x DO Box 2 etOH Friedman Blue SS High CO2 4/5-6/15 release 103B All shells mostly transluscent.
88 FHL Oly OA x DO Box 2 etOH Friedman Pink 44/5-6/15 release FB LCO2 FB 103A

 

Example images from notes above:

 

Sample #74

 

 

Sample #75

 

 

Sample #77

 

 

Sample #85

 

 

Sample #86

 

 

Sample #87

Morphometrics – Olympia Oyster OA Larvae Continued

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Continued measuring Ostrea lurida larvae using NIS-Elements BR (10x objective and 10x nosepiece setting in software).

All measurements can be found here (Google Sheet): FHL_Oly_larvae_measurements

Measured 30 larvae from each tube of the following samples:

  Box Label Color Tube Label Notes
34 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 108B LH-L-1 D5 SS
35 2015 – DO x pH Olys FHL Friedman White 3.24.15 103A-SS Olys. Low CO2 released 3.23.15 Jagged shells; See image below
36 2015 – DO x pH Olys FHL Friedman White 3.26.15 SS LL-H1 103A
37 2015 – DO x pH Olys FHL Friedman White 103A 3.26.15SS LL-H2
38 2015 – DO x pH Olys FHL Friedman White 103B 3.26.15 SS LH-H-1
39 2015 – DO x pH Olys FHL Friedman White 3.26.15 103BSS LHH-2
40 2015 – DO x pH Olys FHL Friedman White 108A 3.26.15 SS LL-L-1
41 2015 – DO x pH Olys FHL Friedman White 108A 3.26.15 SS LL-L-2
42 2015 – DO x pH Olys FHL Friedman White 108B 3.26.15 LHL-1 SS
43 2015 – DO x pH Olys FHL Friedman Purple AR 103B FB Release 3.31.15 Many transluscent shells; See image below
44 2015 – DO x pH Olys FHL Friedman Pink LCO2 FB 103A 2d 3/25/15 release 3.27.15 All transluscent shells; See image below
45 2015 – DO x pH Olys FHL Friedman Blue LCO2 SS 3.27.15 103A from 3/23/15 release All semi-transluscent shells; See image below
46 2015 – DO x pH Olys FHL Friedman Pink 3.27.15 103A FB 3.26.15 released LCO2 – 1 day old
47 2015 – DO x pH Olys FHL Friedman Purple 3.30.15 103B FB HCO2 release
48 2015 – DO x pH Olys FHL Friedman Purple 3.30.15 FB LCO2 release 103A
49 2015 – DO x pH Olys FHL Friedman Pink AS 103A-FB released 3.31.15
50 2015 – DO x pH Olys FHL Friedman Pink FB LCO2-103A released 4.1.15 4.1.15
51 2015 – DO x pH Olys FHL Friedman White SS LCO2-103A released 3.31.15 4.1.15
52 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.GG SS HLH3
53 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.HH SS HLH4
54 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LHH2 4.3.D All semi-transluscent shells; See image below
55 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LLH1 4-3-A
56 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LLH2 4-3-B
57 FHL Oly OA x DO Box 2 etOH Friedman White 4.3.15 SS LHH1 4-3-C
58 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 I HLH-1 SS
59 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 J HLH-2 SS
60 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 K HHH-1 SS
61 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 L HHH-2 SS
62 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 M HLL-1 SS
63 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 N HLL-2 SS
64 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 O HHL-1 SS
65 FHL Oly OA x DO Box 2 etOH Friedman Blue 4.3.15 P HHL-2 SS
66 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 S FB HHH-1
67 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 T FB HHH-2
68 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 Q FB HL.H-1
69 FHL Oly OA x DO Box 2 etOH Friedman Purple 4.3.15 R FB HL.H-2

 

Example images from notes above:

Sample #35

 

 

Normal shell, for comparison to jagged shell image above:

 

Sample #43 – Many transluscent shells

 

Sample #44 – All transluscent shells

 

Sample #45 – Partially transluscent shells

 

Sample #54 – All transluscent shells

Morphometrics – Olympia Oyster OA Larvae Continued

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Continued measurements of larvae samples from yesterday. Measured length (hinge to growing edge) and width of 30 oysters from each tube using NIS-Elements BR (10x objective and 10x nosepiece setting in software).

The measurement data can be found here (Google Sheet): FHL_Oly_larvae_measurements

Here’s the list of tubes that were measured today and their labels:

  Box Label Color Tube Label Notes
14 2015 – DO x pH Olys FHL Friedman White AM 103B-SS LH-H-2 3.31.15
15 2015 – DO x pH Olys FHL Friedman White AL 103B-SSLH-H-1 3.31.15
16 2015 – DO x pH Olys FHL Friedman White AK 3.31.15 103A-SS LL-H-2
17 2015 – DO x pH Olys FHL Friedman White AJ 3.31.15 108A-SS LL-H-1
18 2015 – DO x pH Olys FHL Friedman Blue AG 108A-SS HL-L-2 4.1.15
19 2015 – DO x pH Olys FHL Friedman White 3.30.15 SS-103A LL-H-3 Day 3 3.30.15
20 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS-103A LLH-4 3.30.15
21 2015 – DO x pH Olys FHL Friedman White 3.30.15 SS-103B Day 3 LH-H-3 3.30.15
22 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS 103B LHH-4 Day 3
23 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS-108A LL-L-3 3.30.15
24 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS 108A LLL-4 Day 3
25 2015 – DO x pH Olys FHL Friedman White 3.30.15 SS 108B Day 3 LHL-3 3.30.15
26 2015 – DO x pH Olys FHL Friedman White 3.30.15 Day 3 SS 108B LH-L-4 3.30.15 Day 3
27 2015 – DO x pH Olys FHL Friedman Blue AH 108B-SS HH-L-1 4.1.15
28 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5-103A LL-H1 SS-D5
29 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 103A LL-H-2 D5 SS
30 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 103B LH-H-1 SS D5
31 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5103B LH-H-2 D5 SS
32 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 108A LL-L-1 D5-SS
33 2015 – DO x pH Olys FHL Friedman White 3.29.15 Day 5 108A LL-L-2 -SS D5

 

Morphometrics – Olympia Oyster OA Larvae

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Olympia oyster larvae were measured using NIS Elements-BR (Nikon) imaging software to measure larvae length (hinge to growing edge) and width (10x objective and 10x nosepiece setting on software). Below is a screenshot of what the software, larvae, and measurement controls look like:

Measured 30 individuals from each tube.

The following samples were measured (these are the tube labels; currently not certain what shorthand labelling scheme should be used):

  Box Label Color Tube Label Notes
1 2015 – DO x pH Olys FHL Friedman White/Blue Released: 3.25.15 103B-SS Olys High CO2 3-25-15
2 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103A HLH-1 3.28.15 3/25/release
3 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103A HL-H-2 3.28.15 3.25 release
4 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103B HH-H-1 3.28.15 3/25 release
5 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 103B HH-H-2 3.27.15 release
6 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 108A HL-L-1 3.27.15 3/25 release
7 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 108A HL-L2 3.27.15 3.25 release
8 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 108B HH-L-1 3.27.15 3/25 release
9 2015 – DO x pH Olys FHL Friedman Blue SS Day 3 HH-L-2 3.27.15 3.25 release
10 2015 – DO x pH Olys FHL Friedman Blue AI 108B-SS HH-L-2 4.1.15
11 2015 – DO x pH Olys FHL Friedman White Ap 108B-SS LH-L-1 3.31.15
12 2015 – DO x pH Olys FHL Friedman White AN 108A-SS LL-L-1 3.31.15
13 2015 – DO x pH Olys FHL Friedman White AO 103A-SS LL-L-2 3.31.15

Measurement data is here (Google Sheet): FHL_Oly_larvae_measurements

RNA Isolation – Olympia Oyster Larvae from May 2012

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Isolated RNA from 11 Olympia (O. lurida) oyster larvae samples, from 4 different pCO2 treatments using TriReagent (MRC) according to the manufacturer’s protocol. RNA was resuspended in 100uL of 0.1% DEPC-H2O, spec’d on the Roberts Lab NanoDrop 1000, and stored @ -80C.

Results:

RNA looks good. Will prepare for submission for Illumina HiSeq sequencing. Sample pooling info is here.