I selected the following samples (Ab Endo 2011 Water Filter DNA samples) to send to Alice Nguyen at the Marine Science Institute for digital droplet PCR (ddPCR):
- CI SRI CP 1A (0 copies)
- CI SRI CP 2B (highest)
- CI SRI CP 2A (0 copies)
- CI SRI CP 1B (high)
- CARMEL +500M 1 (medium)
- CARMEL +500M 2 (low)
10μL of each sample was sent. Tubes were labelled with “DNA #”. The ‘#’ corresponds to the number in the list above.
10μL of each of p18RK7 qPCR standards (from 20120730) were sent.
Two sets of WSN1 F/R working stocks (10μM) were also sent.
Also sent the QX200 Droplet Generation Oil for EvaGreen Kit (BioRad) that we ordered.
Shipment was on “wet” ice.