PCR – Withering Syndrome cPCR Taq Test

Ran PCR comparing GoTaq (Promega) and Velocity Taq (Bioline), while also comparing dNTPs (Bioline). Master mix calcs are here. All samples were run in duplicate.

Used the 3e7 copies of the p16RK7 plasmid curve (from 20120731) as template.

Results:

Ladder is Hyperladder V (Bioline).

No amplification with either dNTP set using the newly purchased GoTaq. Oddly, it seems like there’s some amplification (inconsistent, at that) with the Velocity Taq, but the band produced is too large.

Since this didn’t work, I guess I’ll try a new aliquot of the primer working stocks.