This is a qPCR to test the fresh p18RK7 dilution I made earlier today, and verify it works well (i.e. linear spread, good R^2 value, same Cqs as previous curve dilution, etc.).
Master mix calcs were re-used from previous standard curve check (Google Sheet): 20161128 – qPCR WS p18RK7 Curve Check
I made three separate master mixes to check the curve three times.
All samples were run in duplicate. Plate layout, cycling params, etc. are in the qPCR Report (see Results below).
Baseline threshold was manually set to 580, as previously determined by Lisa for this assay.
Curve looks good! Will use this one going forward. Will store in the withering syndrome standard curve box in the FSH 240 4C.